envelope encoding protein Search Results


replication-competent recombinant vesicular stomatitis virus encoding hepatitis c virus envelope proteins  (Japan Tobacco Inc)
90
Japan Tobacco Inc replication-competent recombinant vesicular stomatitis virus encoding hepatitis c virus envelope proteins
Replication Competent Recombinant Vesicular Stomatitis Virus Encoding Hepatitis C Virus Envelope Proteins, supplied by Japan Tobacco Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmids encoding hcv genotype 1a envelope proteins  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare plasmids encoding hcv genotype 1a envelope proteins
(A) Human hepatocytes (Huh7.5.1 cells) were infected with wild-type HCVcc in the presence of the compounds at 10 μM overnight followed by incubation with compound treatment for an additional 48 hours. Viral RNA was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). (B) Primary human hepatocytes were infected with wild-type HCVcc in the presence of (S)-CCZ titration overnight followed by incubation with (S)-CCZ titration for an additional 48 hours. Intracellular viral RNA levels were evaluated by qRTPCR. (C) In the presence of compound treatment, Huh7.5.1 cells were passaged every 3 days for seven passages and were plated on 96-well plates 3 days before ATPlite assay to measure cell viability. (D) <t>HCV</t> replication cycle assays were carried out with (S)-CCZ at 10 μM. Cyclosporin A (10 μM) was a control in HCV single-cycle infection (HCVsc), transient replicon genotype <t>1a,</t> and replicon genotype 1b and 2a assays. Bafilomycin A1 (10 nM) was used as a control in HCV pseudoparticle (HCVpp) genotype 1a and 1b, vesicular stomatitis virus G pseudoparticle (VSV-Gpp), and murine leukemia virus pseudoparticle (MLVpp) assays. Results were normalized to DMSO. RLU, relative luminescence units. GT, genotype. (E) At t = −2 hours, HCV-Luc was incubated with Huh7.5.1 cells at 4°C for 2 hours for attachment. At t =0 hours, the unbound virus was removed, and the plates were moved to 37°C to allow synchronous infection and incubated for 48 hours before virus load measurement. (S)-CCZ (10 μM), bafilomycin A1 (10 nM), and sofosbuvir (10 μM) were added either continuously or at the indicated time points and incubated for 2 hours. (F) Results from (E) were normalized to DMSO continuous treatment. Data are means of replicates ± SEM (n ≥ 3). *P < 0.05, **P < 0.005, ***P < 0.0001, versus DMSO (Student’s t test).
Plasmids Encoding Hcv Genotype 1a Envelope Proteins, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genes encoding the capsid and envelope proteins  (Bio Basic Canada)
90
Bio Basic Canada genes encoding the capsid and envelope proteins
(A) Human hepatocytes (Huh7.5.1 cells) were infected with wild-type HCVcc in the presence of the compounds at 10 μM overnight followed by incubation with compound treatment for an additional 48 hours. Viral RNA was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). (B) Primary human hepatocytes were infected with wild-type HCVcc in the presence of (S)-CCZ titration overnight followed by incubation with (S)-CCZ titration for an additional 48 hours. Intracellular viral RNA levels were evaluated by qRTPCR. (C) In the presence of compound treatment, Huh7.5.1 cells were passaged every 3 days for seven passages and were plated on 96-well plates 3 days before ATPlite assay to measure cell viability. (D) <t>HCV</t> replication cycle assays were carried out with (S)-CCZ at 10 μM. Cyclosporin A (10 μM) was a control in HCV single-cycle infection (HCVsc), transient replicon genotype <t>1a,</t> and replicon genotype 1b and 2a assays. Bafilomycin A1 (10 nM) was used as a control in HCV pseudoparticle (HCVpp) genotype 1a and 1b, vesicular stomatitis virus G pseudoparticle (VSV-Gpp), and murine leukemia virus pseudoparticle (MLVpp) assays. Results were normalized to DMSO. RLU, relative luminescence units. GT, genotype. (E) At t = −2 hours, HCV-Luc was incubated with Huh7.5.1 cells at 4°C for 2 hours for attachment. At t =0 hours, the unbound virus was removed, and the plates were moved to 37°C to allow synchronous infection and incubated for 48 hours before virus load measurement. (S)-CCZ (10 μM), bafilomycin A1 (10 nM), and sofosbuvir (10 μM) were added either continuously or at the indicated time points and incubated for 2 hours. (F) Results from (E) were normalized to DMSO continuous treatment. Data are means of replicates ± SEM (n ≥ 3). *P < 0.05, **P < 0.005, ***P < 0.0001, versus DMSO (Student’s t test).
Genes Encoding The Capsid And Envelope Proteins, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleic acid molecules encoding envelope domain iii proteins  (GenScript corporation)
90
GenScript corporation nucleic acid molecules encoding envelope domain iii proteins
(A) Human hepatocytes (Huh7.5.1 cells) were infected with wild-type HCVcc in the presence of the compounds at 10 μM overnight followed by incubation with compound treatment for an additional 48 hours. Viral RNA was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). (B) Primary human hepatocytes were infected with wild-type HCVcc in the presence of (S)-CCZ titration overnight followed by incubation with (S)-CCZ titration for an additional 48 hours. Intracellular viral RNA levels were evaluated by qRTPCR. (C) In the presence of compound treatment, Huh7.5.1 cells were passaged every 3 days for seven passages and were plated on 96-well plates 3 days before ATPlite assay to measure cell viability. (D) <t>HCV</t> replication cycle assays were carried out with (S)-CCZ at 10 μM. Cyclosporin A (10 μM) was a control in HCV single-cycle infection (HCVsc), transient replicon genotype <t>1a,</t> and replicon genotype 1b and 2a assays. Bafilomycin A1 (10 nM) was used as a control in HCV pseudoparticle (HCVpp) genotype 1a and 1b, vesicular stomatitis virus G pseudoparticle (VSV-Gpp), and murine leukemia virus pseudoparticle (MLVpp) assays. Results were normalized to DMSO. RLU, relative luminescence units. GT, genotype. (E) At t = −2 hours, HCV-Luc was incubated with Huh7.5.1 cells at 4°C for 2 hours for attachment. At t =0 hours, the unbound virus was removed, and the plates were moved to 37°C to allow synchronous infection and incubated for 48 hours before virus load measurement. (S)-CCZ (10 μM), bafilomycin A1 (10 nM), and sofosbuvir (10 μM) were added either continuously or at the indicated time points and incubated for 2 hours. (F) Results from (E) were normalized to DMSO continuous treatment. Data are means of replicates ± SEM (n ≥ 3). *P < 0.05, **P < 0.005, ***P < 0.0001, versus DMSO (Student’s t test).
Nucleic Acid Molecules Encoding Envelope Domain Iii Proteins, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleic acid molecules encoding dengue envelope domain iii proteins  (GenScript corporation)
90
GenScript corporation nucleic acid molecules encoding dengue envelope domain iii proteins
(A) Human hepatocytes (Huh7.5.1 cells) were infected with wild-type HCVcc in the presence of the compounds at 10 μM overnight followed by incubation with compound treatment for an additional 48 hours. Viral RNA was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). (B) Primary human hepatocytes were infected with wild-type HCVcc in the presence of (S)-CCZ titration overnight followed by incubation with (S)-CCZ titration for an additional 48 hours. Intracellular viral RNA levels were evaluated by qRTPCR. (C) In the presence of compound treatment, Huh7.5.1 cells were passaged every 3 days for seven passages and were plated on 96-well plates 3 days before ATPlite assay to measure cell viability. (D) <t>HCV</t> replication cycle assays were carried out with (S)-CCZ at 10 μM. Cyclosporin A (10 μM) was a control in HCV single-cycle infection (HCVsc), transient replicon genotype <t>1a,</t> and replicon genotype 1b and 2a assays. Bafilomycin A1 (10 nM) was used as a control in HCV pseudoparticle (HCVpp) genotype 1a and 1b, vesicular stomatitis virus G pseudoparticle (VSV-Gpp), and murine leukemia virus pseudoparticle (MLVpp) assays. Results were normalized to DMSO. RLU, relative luminescence units. GT, genotype. (E) At t = −2 hours, HCV-Luc was incubated with Huh7.5.1 cells at 4°C for 2 hours for attachment. At t =0 hours, the unbound virus was removed, and the plates were moved to 37°C to allow synchronous infection and incubated for 48 hours before virus load measurement. (S)-CCZ (10 μM), bafilomycin A1 (10 nM), and sofosbuvir (10 μM) were added either continuously or at the indicated time points and incubated for 2 hours. (F) Results from (E) were normalized to DMSO continuous treatment. Data are means of replicates ± SEM (n ≥ 3). *P < 0.05, **P < 0.005, ***P < 0.0001, versus DMSO (Student’s t test).
Nucleic Acid Molecules Encoding Dengue Envelope Domain Iii Proteins, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene encoding the herv envelope protein  (GenScript corporation)
90
GenScript corporation gene encoding the herv envelope protein
(A) Human hepatocytes (Huh7.5.1 cells) were infected with wild-type HCVcc in the presence of the compounds at 10 μM overnight followed by incubation with compound treatment for an additional 48 hours. Viral RNA was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). (B) Primary human hepatocytes were infected with wild-type HCVcc in the presence of (S)-CCZ titration overnight followed by incubation with (S)-CCZ titration for an additional 48 hours. Intracellular viral RNA levels were evaluated by qRTPCR. (C) In the presence of compound treatment, Huh7.5.1 cells were passaged every 3 days for seven passages and were plated on 96-well plates 3 days before ATPlite assay to measure cell viability. (D) <t>HCV</t> replication cycle assays were carried out with (S)-CCZ at 10 μM. Cyclosporin A (10 μM) was a control in HCV single-cycle infection (HCVsc), transient replicon genotype <t>1a,</t> and replicon genotype 1b and 2a assays. Bafilomycin A1 (10 nM) was used as a control in HCV pseudoparticle (HCVpp) genotype 1a and 1b, vesicular stomatitis virus G pseudoparticle (VSV-Gpp), and murine leukemia virus pseudoparticle (MLVpp) assays. Results were normalized to DMSO. RLU, relative luminescence units. GT, genotype. (E) At t = −2 hours, HCV-Luc was incubated with Huh7.5.1 cells at 4°C for 2 hours for attachment. At t =0 hours, the unbound virus was removed, and the plates were moved to 37°C to allow synchronous infection and incubated for 48 hours before virus load measurement. (S)-CCZ (10 μM), bafilomycin A1 (10 nM), and sofosbuvir (10 μM) were added either continuously or at the indicated time points and incubated for 2 hours. (F) Results from (E) were normalized to DMSO continuous treatment. Data are means of replicates ± SEM (n ≥ 3). *P < 0.05, **P < 0.005, ***P < 0.0001, versus DMSO (Student’s t test).
Gene Encoding The Herv Envelope Protein, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna sequence encoding jev envelope protein domain iii  (Eurofins)
90
Eurofins dna sequence encoding jev envelope protein domain iii
(A) Human hepatocytes (Huh7.5.1 cells) were infected with wild-type HCVcc in the presence of the compounds at 10 μM overnight followed by incubation with compound treatment for an additional 48 hours. Viral RNA was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). (B) Primary human hepatocytes were infected with wild-type HCVcc in the presence of (S)-CCZ titration overnight followed by incubation with (S)-CCZ titration for an additional 48 hours. Intracellular viral RNA levels were evaluated by qRTPCR. (C) In the presence of compound treatment, Huh7.5.1 cells were passaged every 3 days for seven passages and were plated on 96-well plates 3 days before ATPlite assay to measure cell viability. (D) <t>HCV</t> replication cycle assays were carried out with (S)-CCZ at 10 μM. Cyclosporin A (10 μM) was a control in HCV single-cycle infection (HCVsc), transient replicon genotype <t>1a,</t> and replicon genotype 1b and 2a assays. Bafilomycin A1 (10 nM) was used as a control in HCV pseudoparticle (HCVpp) genotype 1a and 1b, vesicular stomatitis virus G pseudoparticle (VSV-Gpp), and murine leukemia virus pseudoparticle (MLVpp) assays. Results were normalized to DMSO. RLU, relative luminescence units. GT, genotype. (E) At t = −2 hours, HCV-Luc was incubated with Huh7.5.1 cells at 4°C for 2 hours for attachment. At t =0 hours, the unbound virus was removed, and the plates were moved to 37°C to allow synchronous infection and incubated for 48 hours before virus load measurement. (S)-CCZ (10 μM), bafilomycin A1 (10 nM), and sofosbuvir (10 μM) were added either continuously or at the indicated time points and incubated for 2 hours. (F) Results from (E) were normalized to DMSO continuous treatment. Data are means of replicates ± SEM (n ≥ 3). *P < 0.05, **P < 0.005, ***P < 0.0001, versus DMSO (Student’s t test).
Dna Sequence Encoding Jev Envelope Protein Domain Iii, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid dna encoding beta-galactosidase fused to an envelope protein of endogenous retrovirus  (Takeda)
90
Takeda plasmid dna encoding beta-galactosidase fused to an envelope protein of endogenous retrovirus
(A) Human hepatocytes (Huh7.5.1 cells) were infected with wild-type HCVcc in the presence of the compounds at 10 μM overnight followed by incubation with compound treatment for an additional 48 hours. Viral RNA was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). (B) Primary human hepatocytes were infected with wild-type HCVcc in the presence of (S)-CCZ titration overnight followed by incubation with (S)-CCZ titration for an additional 48 hours. Intracellular viral RNA levels were evaluated by qRTPCR. (C) In the presence of compound treatment, Huh7.5.1 cells were passaged every 3 days for seven passages and were plated on 96-well plates 3 days before ATPlite assay to measure cell viability. (D) <t>HCV</t> replication cycle assays were carried out with (S)-CCZ at 10 μM. Cyclosporin A (10 μM) was a control in HCV single-cycle infection (HCVsc), transient replicon genotype <t>1a,</t> and replicon genotype 1b and 2a assays. Bafilomycin A1 (10 nM) was used as a control in HCV pseudoparticle (HCVpp) genotype 1a and 1b, vesicular stomatitis virus G pseudoparticle (VSV-Gpp), and murine leukemia virus pseudoparticle (MLVpp) assays. Results were normalized to DMSO. RLU, relative luminescence units. GT, genotype. (E) At t = −2 hours, HCV-Luc was incubated with Huh7.5.1 cells at 4°C for 2 hours for attachment. At t =0 hours, the unbound virus was removed, and the plates were moved to 37°C to allow synchronous infection and incubated for 48 hours before virus load measurement. (S)-CCZ (10 μM), bafilomycin A1 (10 nM), and sofosbuvir (10 μM) were added either continuously or at the indicated time points and incubated for 2 hours. (F) Results from (E) were normalized to DMSO continuous treatment. Data are means of replicates ± SEM (n ≥ 3). *P < 0.05, **P < 0.005, ***P < 0.0001, versus DMSO (Student’s t test).
Plasmid Dna Encoding Beta Galactosidase Fused To An Envelope Protein Of Endogenous Retrovirus, supplied by Takeda, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic gene encoding domain iii of envelope protein of dengue virus type 4  (Biotech Desk Pvt Ltd)
90
Biotech Desk Pvt Ltd synthetic gene encoding domain iii of envelope protein of dengue virus type 4
(A) Human hepatocytes (Huh7.5.1 cells) were infected with wild-type HCVcc in the presence of the compounds at 10 μM overnight followed by incubation with compound treatment for an additional 48 hours. Viral RNA was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). (B) Primary human hepatocytes were infected with wild-type HCVcc in the presence of (S)-CCZ titration overnight followed by incubation with (S)-CCZ titration for an additional 48 hours. Intracellular viral RNA levels were evaluated by qRTPCR. (C) In the presence of compound treatment, Huh7.5.1 cells were passaged every 3 days for seven passages and were plated on 96-well plates 3 days before ATPlite assay to measure cell viability. (D) <t>HCV</t> replication cycle assays were carried out with (S)-CCZ at 10 μM. Cyclosporin A (10 μM) was a control in HCV single-cycle infection (HCVsc), transient replicon genotype <t>1a,</t> and replicon genotype 1b and 2a assays. Bafilomycin A1 (10 nM) was used as a control in HCV pseudoparticle (HCVpp) genotype 1a and 1b, vesicular stomatitis virus G pseudoparticle (VSV-Gpp), and murine leukemia virus pseudoparticle (MLVpp) assays. Results were normalized to DMSO. RLU, relative luminescence units. GT, genotype. (E) At t = −2 hours, HCV-Luc was incubated with Huh7.5.1 cells at 4°C for 2 hours for attachment. At t =0 hours, the unbound virus was removed, and the plates were moved to 37°C to allow synchronous infection and incubated for 48 hours before virus load measurement. (S)-CCZ (10 μM), bafilomycin A1 (10 nM), and sofosbuvir (10 μM) were added either continuously or at the indicated time points and incubated for 2 hours. (F) Results from (E) were normalized to DMSO continuous treatment. Data are means of replicates ± SEM (n ≥ 3). *P < 0.05, **P < 0.005, ***P < 0.0001, versus DMSO (Student’s t test).
Synthetic Gene Encoding Domain Iii Of Envelope Protein Of Dengue Virus Type 4, supplied by Biotech Desk Pvt Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene encoding nuclear envelope protein lamin a/c or lmna  (Blackwell Science Ltd)
90
Blackwell Science Ltd gene encoding nuclear envelope protein lamin a/c or lmna
(A) Human hepatocytes (Huh7.5.1 cells) were infected with wild-type HCVcc in the presence of the compounds at 10 μM overnight followed by incubation with compound treatment for an additional 48 hours. Viral RNA was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). (B) Primary human hepatocytes were infected with wild-type HCVcc in the presence of (S)-CCZ titration overnight followed by incubation with (S)-CCZ titration for an additional 48 hours. Intracellular viral RNA levels were evaluated by qRTPCR. (C) In the presence of compound treatment, Huh7.5.1 cells were passaged every 3 days for seven passages and were plated on 96-well plates 3 days before ATPlite assay to measure cell viability. (D) <t>HCV</t> replication cycle assays were carried out with (S)-CCZ at 10 μM. Cyclosporin A (10 μM) was a control in HCV single-cycle infection (HCVsc), transient replicon genotype <t>1a,</t> and replicon genotype 1b and 2a assays. Bafilomycin A1 (10 nM) was used as a control in HCV pseudoparticle (HCVpp) genotype 1a and 1b, vesicular stomatitis virus G pseudoparticle (VSV-Gpp), and murine leukemia virus pseudoparticle (MLVpp) assays. Results were normalized to DMSO. RLU, relative luminescence units. GT, genotype. (E) At t = −2 hours, HCV-Luc was incubated with Huh7.5.1 cells at 4°C for 2 hours for attachment. At t =0 hours, the unbound virus was removed, and the plates were moved to 37°C to allow synchronous infection and incubated for 48 hours before virus load measurement. (S)-CCZ (10 μM), bafilomycin A1 (10 nM), and sofosbuvir (10 μM) were added either continuously or at the indicated time points and incubated for 2 hours. (F) Results from (E) were normalized to DMSO continuous treatment. Data are means of replicates ± SEM (n ≥ 3). *P < 0.05, **P < 0.005, ***P < 0.0001, versus DMSO (Student’s t test).
Gene Encoding Nuclear Envelope Protein Lamin A/C Or Lmna, supplied by Blackwell Science Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdc plasmid encoding wnv premembrane and envelope proteins in the form of a subviral particle  (Abbott Laboratories)
90
Abbott Laboratories cdc plasmid encoding wnv premembrane and envelope proteins in the form of a subviral particle
(A) Human hepatocytes (Huh7.5.1 cells) were infected with wild-type HCVcc in the presence of the compounds at 10 μM overnight followed by incubation with compound treatment for an additional 48 hours. Viral RNA was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). (B) Primary human hepatocytes were infected with wild-type HCVcc in the presence of (S)-CCZ titration overnight followed by incubation with (S)-CCZ titration for an additional 48 hours. Intracellular viral RNA levels were evaluated by qRTPCR. (C) In the presence of compound treatment, Huh7.5.1 cells were passaged every 3 days for seven passages and were plated on 96-well plates 3 days before ATPlite assay to measure cell viability. (D) <t>HCV</t> replication cycle assays were carried out with (S)-CCZ at 10 μM. Cyclosporin A (10 μM) was a control in HCV single-cycle infection (HCVsc), transient replicon genotype <t>1a,</t> and replicon genotype 1b and 2a assays. Bafilomycin A1 (10 nM) was used as a control in HCV pseudoparticle (HCVpp) genotype 1a and 1b, vesicular stomatitis virus G pseudoparticle (VSV-Gpp), and murine leukemia virus pseudoparticle (MLVpp) assays. Results were normalized to DMSO. RLU, relative luminescence units. GT, genotype. (E) At t = −2 hours, HCV-Luc was incubated with Huh7.5.1 cells at 4°C for 2 hours for attachment. At t =0 hours, the unbound virus was removed, and the plates were moved to 37°C to allow synchronous infection and incubated for 48 hours before virus load measurement. (S)-CCZ (10 μM), bafilomycin A1 (10 nM), and sofosbuvir (10 μM) were added either continuously or at the indicated time points and incubated for 2 hours. (F) Results from (E) were normalized to DMSO continuous treatment. Data are means of replicates ± SEM (n ≥ 3). *P < 0.05, **P < 0.005, ***P < 0.0001, versus DMSO (Student’s t test).
Cdc Plasmid Encoding Wnv Premembrane And Envelope Proteins In The Form Of A Subviral Particle, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Human hepatocytes (Huh7.5.1 cells) were infected with wild-type HCVcc in the presence of the compounds at 10 μM overnight followed by incubation with compound treatment for an additional 48 hours. Viral RNA was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). (B) Primary human hepatocytes were infected with wild-type HCVcc in the presence of (S)-CCZ titration overnight followed by incubation with (S)-CCZ titration for an additional 48 hours. Intracellular viral RNA levels were evaluated by qRTPCR. (C) In the presence of compound treatment, Huh7.5.1 cells were passaged every 3 days for seven passages and were plated on 96-well plates 3 days before ATPlite assay to measure cell viability. (D) HCV replication cycle assays were carried out with (S)-CCZ at 10 μM. Cyclosporin A (10 μM) was a control in HCV single-cycle infection (HCVsc), transient replicon genotype 1a, and replicon genotype 1b and 2a assays. Bafilomycin A1 (10 nM) was used as a control in HCV pseudoparticle (HCVpp) genotype 1a and 1b, vesicular stomatitis virus G pseudoparticle (VSV-Gpp), and murine leukemia virus pseudoparticle (MLVpp) assays. Results were normalized to DMSO. RLU, relative luminescence units. GT, genotype. (E) At t = −2 hours, HCV-Luc was incubated with Huh7.5.1 cells at 4°C for 2 hours for attachment. At t =0 hours, the unbound virus was removed, and the plates were moved to 37°C to allow synchronous infection and incubated for 48 hours before virus load measurement. (S)-CCZ (10 μM), bafilomycin A1 (10 nM), and sofosbuvir (10 μM) were added either continuously or at the indicated time points and incubated for 2 hours. (F) Results from (E) were normalized to DMSO continuous treatment. Data are means of replicates ± SEM (n ≥ 3). *P < 0.05, **P < 0.005, ***P < 0.0001, versus DMSO (Student’s t test).

Journal: Science translational medicine

Article Title: Repurposing of the antihistamine chlorcyclizine and related compounds for treatment of hepatitis C virus infection

doi: 10.1126/scitranslmed.3010286

Figure Lengend Snippet: (A) Human hepatocytes (Huh7.5.1 cells) were infected with wild-type HCVcc in the presence of the compounds at 10 μM overnight followed by incubation with compound treatment for an additional 48 hours. Viral RNA was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). (B) Primary human hepatocytes were infected with wild-type HCVcc in the presence of (S)-CCZ titration overnight followed by incubation with (S)-CCZ titration for an additional 48 hours. Intracellular viral RNA levels were evaluated by qRTPCR. (C) In the presence of compound treatment, Huh7.5.1 cells were passaged every 3 days for seven passages and were plated on 96-well plates 3 days before ATPlite assay to measure cell viability. (D) HCV replication cycle assays were carried out with (S)-CCZ at 10 μM. Cyclosporin A (10 μM) was a control in HCV single-cycle infection (HCVsc), transient replicon genotype 1a, and replicon genotype 1b and 2a assays. Bafilomycin A1 (10 nM) was used as a control in HCV pseudoparticle (HCVpp) genotype 1a and 1b, vesicular stomatitis virus G pseudoparticle (VSV-Gpp), and murine leukemia virus pseudoparticle (MLVpp) assays. Results were normalized to DMSO. RLU, relative luminescence units. GT, genotype. (E) At t = −2 hours, HCV-Luc was incubated with Huh7.5.1 cells at 4°C for 2 hours for attachment. At t =0 hours, the unbound virus was removed, and the plates were moved to 37°C to allow synchronous infection and incubated for 48 hours before virus load measurement. (S)-CCZ (10 μM), bafilomycin A1 (10 nM), and sofosbuvir (10 μM) were added either continuously or at the indicated time points and incubated for 2 hours. (F) Results from (E) were normalized to DMSO continuous treatment. Data are means of replicates ± SEM (n ≥ 3). *P < 0.05, **P < 0.005, ***P < 0.0001, versus DMSO (Student’s t test).

Article Snippet: Plasmids encoding HCV genotype 1a envelope proteins were provided by S. Ray (Johns Hopkins University).

Techniques: Infection, Incubation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Titration, Control, Virus